How to submit samples to the AFTOL project
Contact one of the coordinating Principal Investigators. The following people are responsible for sampling of taxa as part of AFTOL.Molecular data:
- David Hibbett: Basidiomycota
- Francois Lutzoni: lichenized Ascomycota
- Joey Spatafora: nonlichenized Ascomycota
- Rytas Vilgalys: Zygomycota/Chytridiomycota
- David McLaughlin: all taxa
Before submitting samples for the AFTOL project, please contact the appropriate PI regarding the taxa you would like to contribute. Coordination will be required with respect to the taxonomy and selection of exemplar taxa. The taxon sampling lists for each of the four taxonomic groups listed above will be compiled by the coordinating PIs in consultation the fungal systematic community. Preliminary lists are available at the AFTOL websites. Now is the time to review the list, make firm suggestions about what you can contribute, and coordinate the shipment of specimens to the appropriate PI.
- Submitting specimens
Specimens can be submitted either as cultures, sporocarps, DNA, or PCR products. Prior to sending the specimen, please coordinate with the appropriate PI. Permits may be necessary for shipment of some material and therefore the necessary documentation will need to be obtained.
- Cultures: check with the PI to see if permits are necessary
- Sporocarps: check with the PI to determine the most appropriate method of specimen preservation, if applicable, and the best form of shipment
- DNAs and PCR products: check with the PI to determine the most appropriate method of shipment (e.g., lyophilized, frozen, etc.)
- Sequencing of specimens
Once specimens have been received, the target loci will be amplified and sequenced. Once sequences have been proofed, they will immediately posted in the AFTOL web-accessible database and returned to the person/laboratory that provided the specimen.
- Sampling of subcellular characters
If you are interested in providing specimens for sampling of subcellular characters please contact David McLaughlin. He is compiling a list of target taxa. Importantly, taxa that are sampled for subcellular characters must also be sampled for molecular data. Therefore, after David has confirmed that a specimen will be sampled for subcellular characters, he will initiate contact between the person contributing the specimen and the appropriate PI responsible for coordinating the collection of molecular data. The contributor can i) send splits of the specimen to both David McLaughlin and the appropriate PI, ii) to the PI coordinating the sampling of molecular data who will forward material to David McLaughlin for sampling of subcellular characters, or iii) to David McLaughlin who will forward material to the appropriate PI for collection of molecular data. Whatever scenario work best for you. In any case, we want to make sure that for any specimen for which subcellular characters are sampled, molecular data are also sampled.
All samples included in the AFTOL project must be vouchered in a recognized herbarium and/or culture collection located at an academic institution, museum, or government facility. The vouchering of specimens into collections can be done by the person providing the specimen or if so desired by the AFTOL PI coordinating the sampling of the specimen. In the former case, the voucher information (e.g., herbarium name and accession number) must be provided with the specimen. In the latter case, the voucher information will be forwarded to the donor of the specimen by the AFTOL PI. In both cases, the voucher information will be made available along with the sequence data in the AFTOL database.
- You are interested in providing specimens for the Basidiomycota. You review the Basidiomycota target list of specimens and contact David Hibbett. You make suggestions for taxa to sample and agree to provide certain species. If permits are needed, David will initiate the process of obtaining the appropriate permit. You also contact David McLaughlin and suggest that one of the specimens you will be providing to David Hibbett should be included in the collection of subcellular characters. Let's use Pleurotus populinus as an example.
- You have made a collection of P. populinus complete with good collection notes and have established a culture. You would then contact David and let him know that you have the specimen of P. populinus ready to send. You and David would then establish the most appropriate way to send the specimen (e.g., UPS 2day) and the specimen would be shipped.
- David would receive the specimen and it would be processed for sampling of molecular data. David will also insure that a split of the collection or a duplicate of the culture is forwarded to David McLaughlin for sampling of subcellular characters. After the molecular data have been collected, the sequences will be forwarded to you and deposited in both the AFTOL database and Genbank.
- After the subcellular data have been collected, they will forwarded to you and deposited in the AFTOL database.
- A voucher or perhaps duplicate vouchers of the collection are deposited in the herbaria of Clark University and University of Minnesota. Cultures are deposited at NRRL.
If you have any additional questions regarding the submission of samples for inclusion in AFTOL project, please contact the appropriate PI with respect to the taxa you wish to contribute.